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stemspan sfem ii supplemented with stemspan erythroid expansion medium  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc stemspan sfem ii supplemented with stemspan erythroid expansion medium
    Stemspan Sfem Ii Supplemented With Stemspan Erythroid Expansion Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemspan+sfem+ii+supplemented+with+stemspan+erythroid+expansion+medium/erythroid+differentiation+media+stemspan+sfem+ii+supplemented+with+1%C3%97+stemspan+erythroid+expansion+supplement/pm35489268-35-10-14
    Average 90 stars, based on 1 article reviews
    stemspan sfem ii supplemented with stemspan erythroid expansion medium - by Bioz Stars, 2026-09
    90/100 stars

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    Isolation:

    Article Title: Generation of an induced pluripotent stem cell line from a patient with Angelman syndrome carrying UBE3A mutation.
    Article Snippet: PBMCs were isolated from 5 mL peripheral blood of patient by using SepMate-50 (STEMCELL Technologies) according to manufacturer’s instructions. .. Isolated PBMCs were cultured in StemSpan SFEM II supplemented with StemSpan Erythroid Expansion Medium (STEMCELL Technologies) a days for erythroid cells enrichment and expansion. .. After a week of cell culture, human cDNAs for reprogramming factors (Oct4, Sox2, Lin28, L-Myc, Klf4) and additional improvements were transduced in PBMCs (P3) with episomal vectors pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mP53DD and pCXB-EBNA1 (Thermo Fisher Scientific, A15960) using an Amaxa P3 Primary Cell 4D Nucleofector X Kit (LONZA) and program E0-100.

    Cell Culture:

    Article Title: Generation of an induced pluripotent stem cell line from a patient with Angelman syndrome carrying UBE3A mutation.
    Article Snippet: PBMCs were isolated from 5 mL peripheral blood of patient by using SepMate-50 (STEMCELL Technologies) according to manufacturer’s instructions. .. Isolated PBMCs were cultured in StemSpan SFEM II supplemented with StemSpan Erythroid Expansion Medium (STEMCELL Technologies) a days for erythroid cells enrichment and expansion. .. After a week of cell culture, human cDNAs for reprogramming factors (Oct4, Sox2, Lin28, L-Myc, Klf4) and additional improvements were transduced in PBMCs (P3) with episomal vectors pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mP53DD and pCXB-EBNA1 (Thermo Fisher Scientific, A15960) using an Amaxa P3 Primary Cell 4D Nucleofector X Kit (LONZA) and program E0-100.



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